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Micro-shock wave assisted bacterial transformation

Divya Prakash, G and Rakesh, SG and Chakravortty, Dipshikha and Jagadeesh, G and Karaba, Nataraja (2012) Micro-shock wave assisted bacterial transformation. In: 28th International Symposium on Shock Waves, 2011, Heidelberg.

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Official URL: http://dx.doi.org/10.1007/978-3-642-25685-1_153


A gene is a unit of heredity in a living organism. It normally resides on a stretch of DNA that codes for a type of protein or for an RNA chain that has a function in the organism. All living things depend on genes, as they specify all proteins and functional RNA chains. Genes hold the information to build and maintain an organism’s cells and pass genetic traits to offspring. The gene has to be transferred to bacteria or eukaryotic cells for basic and applied molecular biology studies. Bacteria can uptake exogenous genetic material by three ways: conjugation, transduction and transformation. Genetic material is naturally transferred to bacteria in case of conjugation and transferred through bacteriophage in transduction. Transformation is the acquisition of exogenous genetic material through cell wall. The ability of bacteria of being transformed is called competency and those bacteria which have competency are competent cells. Divalent Calcium ions can make the bacteria competent and a heat shock can cause the bacteria to uptake DNA. But the heat shock method cannot be used for all the bacteria. In electroporation, a brief electric shock with an electric field of 10-20kV/cmmakes pores in the cell wall, facilitates the DNA to enter into the bacteria. Microprecipitates, microinjection, liposomes, and biological vectors are also used to transfer polar molecules like DNA into host cells.

Item Type: Conference Paper
Publisher: Springer-Verlag
Additional Information: Copyright of this article belongs to Springer-Verlag.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Division of Mechanical Sciences > Aerospace Engineering(Formerly Aeronautical Engineering)
Date Deposited: 02 May 2013 09:29
Last Modified: 02 May 2013 09:29
URI: http://eprints.iisc.ac.in/id/eprint/46283

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