A soluble diacylglycerol acyltransferase is involved in triacylglycerol biosynthesis in the oleaginous yeast Rhodotorula glutinis

Rani, Sapa Hima and Saha, Saikat and Rajasekharan, Ram (2013) A soluble diacylglycerol acyltransferase is involved in triacylglycerol biosynthesis in the oleaginous yeast Rhodotorula glutinis. In: MICROBIOLOGY, 159 (Part 1). pp. 155-166.

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Abstract

The biosynthesis of triacylglycerol (TAG) occurs in the microsomal membranes of eukaryotes. Here, we report the identification and functional characterization of diacylglycerol acyltransferase (DGAT), a member of the 10 S cytosolic TAG biosynthetic complex (TBC) in Rhodotorula glutinis. Both a full-length and an N-terminally truncated cDNA clone of a single gene were isolated from R. glutinis. The DGAT activity of the protein encoded by RgDGAT was confirmed in vivo by the heterologous expression of cDNA in a Saccharomyces cerevisiae quadruple mutant (H1246) that is defective in TAG synthesis. RgDGAT overexpression in yeast was found to be capable of acylating diacylglycerol (DAG) in an acyl-CoA-dependent manner. Quadruple mutant yeast cells exhibit growth defects in the presence of oleic acid, but wild-type yeast cells do not. In an in vivo fatty acid supplementation experiment, RgDGAT expression rescued quadruple mutant growth in an oleate-containing medium. We describe a soluble acyl-CoA-dependent DAG acyltransferase from R. glutinis that belongs to the DGAT3 class of enzymes. The study highlights the importance of an alternative TAG biosynthetic pathway in oleaginous yeasts.

Item Type:	Journal Article
Publication:	MICROBIOLOGY
Publisher:	SOC GENERAL MICROBIOLOGY
Additional Information:	Copyrifgt for this article belongs to the Society for General Microbiology
Keywords:	LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE; SACCHAROMYCES- CEREVISIAE; MYCOBACTERIUM-TUBERCULOSIS; PHOSPHOLIPID-SYNTHESIS; ARABIDOPSIS-THALIANA; MOLECULAR-CLONING; ESCHERICHIA-COLI; KEY ENZYME; GENE; ACCUMULATION
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	02 Apr 2013 10:16
Last Modified:	02 Apr 2013 10:16
URI:	http://eprints.iisc.ac.in/id/eprint/46254

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