Structure of SAICAR synthetase from Pyrococcus horikoshii OT3: Insights into thermal stability

Manjunath, Kavyashree and Kanaujia, Shankar Prasad and Kanagaraj, Surekha and Jeyakanthan, Jeyaraman and Sekar, Kanagaraj (2013) Structure of SAICAR synthetase from Pyrococcus horikoshii OT3: Insights into thermal stability. In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 53 . pp. 7-19.

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Abstract

The first native crystal structure of Phosphoribosylaminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) from a hyperthermophilic organism Pyrococcus horikoshii OT3 was determined in two space groups H3 (Type-1: Resolution 2.35 angstrom) and in C222(1) (Type-2: Resolution 1.9 angstrom). Both are dimeric but Type-1 structure exhibited hexameric arrangement due to the presence of cadmium ions. A comparison has been made on the sequence and structures of all SAICAR synthetases to better understand the differences between mesophilic, thermophilic and hyperthermophilic SAICAR synthetases. These SAICAR synthetases are reasonably similar in sequence and three-dimensional structure; however, differences were visible only in the subtler details of percentage composition of the sequences, salt bridge interactions and non-polar contact areas. (c) 2012 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	Copyright for this article belongs to ELSEVIER SCIENCE BV, NETHERLANDS
Keywords:	SAICAR synthetase;Purine de novo biosynthesis;Pyrococcus horikoshii OT3;Hyperthermophile;Thermostable proteins
Department/Centre:	Division of Interdisciplinary Sciences > Supercomputer Education & Research Centre
Date Deposited:	21 Feb 2013 10:41
Last Modified:	21 Feb 2013 10:41
URI:	http://eprints.iisc.ac.in/id/eprint/45841

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