Stiffness- and wettability-dependent myoblast cell compatibility of transparent poly(vinyl alcohol) hydrogels

Gupta, Siddhi and Greeshma, T and Basu, Bikramjit and Goswami, Sudipta and Sinha, Arvind (2013) Stiffness- and wettability-dependent myoblast cell compatibility of transparent poly(vinyl alcohol) hydrogels. In: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 101B (2). pp. 346-354.

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Abstract

This study reports the in vitro compatibility of muscle cells (C2C12 mouse myoblast cell line) with the transparent poly(vinyl alcohol) (PVA) hydrogels and the results are explained on the basis of surface wettability, crystallinity, and nanoscale elastic stiffness property. Nanoindentation was carried out with a maximum load of 100 mu N for all the hydrogel compositions and the properties such as elastic stiffness, hardness and total work done during indentation were computed. The difference in cell viability as well as adhesion of cultured myoblast cells on the investigated hydrogel substrates were discussed in reference to the difference in the nanoscale elastic properties, crystallinity, and surface wettability. An important result has been that both elastic stiffness and surface wettability synergistically influence myoblast viability/adhesion on PVA hydrogels. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.

Item Type:	Journal Article
Publication:	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Publisher:	WILEY-BLACKWELL
Additional Information:	Copyright for this article belongs to WILEY-BLACKWELL, USA
Keywords:	poly(vinyl alcohol);nanoindentation;elastic stiffness;myoblasts
Department/Centre:	Division of Chemical Sciences > Materials Research Centre
Date Deposited:	18 Feb 2013 08:52
Last Modified:	18 Feb 2013 08:52
URI:	http://eprints.iisc.ac.in/id/eprint/45762

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