Oxidative DNA cleavage, cytotoxicity and antimicrobial studies of L-ornithine copper (II) complexes

Chetana, PR and Rao, Ramakrishna and Saha, Sounik and Policegoudra, RS and Vijayan, P and Aradhya, MS (2012) Oxidative DNA cleavage, cytotoxicity and antimicrobial studies of L-ornithine copper (II) complexes. In: POLYHEDRON, 48 (1). pp. 43-50.

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Abstract

New ternary copper (II) complexes, Cu(L-orn)(B)(Cl)](Cl center dot 2H(2)O) (1-2) where L-orn is L-ornithine, B is an N,N-donor heterocyclic base, viz. 2,2'-bipyridine (bpy, 1) and 1,10-phenanthroline (phen, 2), were synthesized and characterized by various spectroscopic techniques. Complex 2 is characterized by the X-ray single crystallographic method. The complex shows a distorted square-pyramidal (4 + 1) CuN3OCl coordination sphere. Binding interactions of the complexes with calf thymus DNA (CT-DNA) were investigated by UV-Vis absorption titration, ethidium bromide displacement assay, viscometric titration experiment and DNA melting studies. Complex 2 shows appreciable chemical nuclease activity in the presence of 3-mercaptopropionic acid (MPA). The complexes were subjected to in vitro cytotoxicity studies against carcinomic human alveolar basal epithelial cells (A-549) and human epithelial (HEp-2) cells. The IC50 values of 1 and 2 are less than that of cisplatin against HEp-2 cell lines. MIC values for 1 against the bacterial strains Streptococcus mutans and Pseudomonas aeruginosa are 0.5 mM. (C) 2012 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Publication:	POLYHEDRON
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Additional Information:	Copyright for this article belongs to PERGAMON-ELSEVIER SCIENCE LTD,ENGLAND
Keywords:	Ornithine;Copper (II);DNA cleavage;A-549;HEp-2;Antibacterial activity
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	16 Jan 2013 12:44
Last Modified:	16 Jan 2013 12:44
URI:	http://eprints.iisc.ac.in/id/eprint/45644

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