Context dependent splicing functions of Bud31/Ycr063w define its role in budding and cell cycle progression

Saha, Debjani and Banerjee, Shataparna and Bashir, Samirul and Vijayraghavan, Usha (2012) Context dependent splicing functions of Bud31/Ycr063w define its role in budding and cell cycle progression. In: Biochemical and Biophysical Research Communications, 424 (3). pp. 579-585.

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Abstract

The yeast Bud31 protein, a Prp19 complex (NTC) member, aids spliceosome assembly and thus promotes efficient pre-mRNA splicing. The bud31 null cells show mild budding abnormalities at optimal growth temperatures and, at higher temperatures, have growth defects with aberrant budding. Here we have assessed cell cycle transitions which require Bud31. We find Bud31 facilitates passage through G1-S regulatory point (Start) but is not needed for G2-M transition or for exit from mitosis. To co-relate Bud31 functions in cell division with splicing, we studied the splicing status of transcripts that encode proteins involved in budding. We find Bud31 promotes efficient splicing of only some of these pre-mRNAs, for example, ARP2 and SRC1. Wild type cells have a long and a short isoform of SRC1 mRNA and protein, out of which the shorter mRNA splice variant is predominant. bud31 Delta cells show inefficient SRC1 splicing and entirely lack the shorter SRC1 spliced mRNA isoform. Yeast PRP17, another NTC sub-complex member, is also required for G1-S and G2-M cell cycle transitions. We examined genetic interactions between BUD31 and PRP17. While both factors were needed for efficient cell cycle dependent gene expression, our data indicate that distinct pre-mRNAs depend on each of these non-essential splicing factors.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science. USA
Keywords:	Bud31;Yeast;S Transition;Budding;Pre-mRNA Splicing
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	28 Feb 2013 11:03
Last Modified:	28 Feb 2013 11:03
URI:	http://eprints.iisc.ac.in/id/eprint/45404

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