ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Severe diffraction anisotropy, rotational pseudosymmetry and twinning complicate the refinement of a pentameric coiled-coil structure of NSP4 of rotavirus

Chacko, Anita R and Zwart, Peter H and Read, Randy J and Dodson, Eleanor J and Rao, CD and Suguna, Kaza (2012) Severe diffraction anisotropy, rotational pseudosymmetry and twinning complicate the refinement of a pentameric coiled-coil structure of NSP4 of rotavirus. In: Acta Crystallographica Section D Biological Crystallography, 68 (11). pp. 1541-1548.

[img] PDF
acta_cry_D_68-11_cha_2012.pdf - Published Version
Restricted to Registered users only

Download (738kB) | Request a copy
Official URL: http://dx.doi.org/10.1107/S090744491203836X

Abstract

The crystal structure of the region spanning residues 95-146 of the rotavirus nonstructural protein NSP4 from the asymptomatic human strain ST3 was determined at a resolution of 2.5 angstrom. Severe diffraction anisotropy, rotational pseudo-symmetry and twinning complicated the refinement of this structure. A systematic explanation confirming the crystal pathologies and describing how the structure was successfully refined is given in this report.

Item Type: Journal Article
Publication: Acta Crystallographica Section D Biological Crystallography
Publisher: International Union of Crystallography
Additional Information: Copyright of this article belongs to International Union of Crystallography.
Keywords: Data Pathology; Diffraction Anisotropy; Rotational Pseudosymmetry; Twinning
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 18 Feb 2013 07:51
Last Modified: 18 Feb 2013 07:51
URI: http://eprints.iisc.ac.in/id/eprint/45367

Actions (login required)

View Item View Item