Non-covalent functionalization using lithographically patterned polyelectrolyte multilayers for high-density microarrays

Prashanth, Gurusiddappa R and Goudar, Venkanagouda S and Suran, Swathi and Raichur, Ashok M and Varma, Manoj M (2012) Non-covalent functionalization using lithographically patterned polyelectrolyte multilayers for high-density microarrays. In: SENSORS AND ACTUATORS B-CHEMICAL, 171 . pp. 315-322.

PDF sen_act_171-172_315_2012.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

We describe a method to fabricate high-density biological microarrays using lithographic patterning of polyelectrolyte multi layers formed by spin assisted electrostatic layer-by-layer assembly. Proteins or DNA can be immobilized on the polyelectrolyte patterns via electrostatic attachment leading to functional microarrays. As the immobilization is done using electrostatically assembled polyelectrolyte anchor, this process is substrate independent and is fully compatible with a standard semiconductor fabrication process flow. Moreover, the electrostatic assembly of the anchor layer is a fast process with reaction saturation times of the order of a few minutes unlike covalent schemes that typically require hours to reach saturation. The substrate independent nature of this technique is demonstrated by functionalizing glass slides as well as regular transparency sheets using the same procedure. Using a model protein assay, we demonstrate that the non-covalent immobilization scheme described here has competitive performance compared to conventional covalent immobilization schemes described in literature. (C) 2012 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	SENSORS AND ACTUATORS B-CHEMICAL
Publisher:	ELSEVIER SCIENCE SA
Additional Information:	Copyright for this article belongs to Elsevier Science
Keywords:	Polyelectrolytes; Non covalent functionalization; High density microarrays; Lithographic bio-patterning
Department/Centre:	Division of Electrical Sciences > Electrical Communication Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy) Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	29 Jan 2013 12:29
Last Modified:	29 Jan 2013 12:29
URI:	http://eprints.iisc.ac.in/id/eprint/45290

Actions (login required)

View Item