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Growth of Polypyrrole-Horseradish Peroxidase Microstructures for H2O2 Biosensor

Kathuroju, Pavan Kumar and Jampana, Nagaraju (2012) Growth of Polypyrrole-Horseradish Peroxidase Microstructures for H2O2 Biosensor. In: Sensors Applications Symposium (SAS), 2011 IEEE, 22-24 Feb. 2011, San Antonio, TX, USA, pp. 2339-2345.

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Official URL: http://dx.doi.org/10.1109/TIM.2012.2199198


Conducting polymer microstructures for enzymatic biosensors are developed by a facile electrochemical route. Horseradish peroxide (HRP)-entrapped polypyrrole (PPy) films with bowl-shaped microstructures are developed on stainless steel (SS 304) substrates by a single-step process. Potentiodynamic scanning/cyclic voltammetry is used for generation of PPy microstructures using electrogenerated oxygen bubbles stabilized by zwitterionic surfactant/buffer N-2-hydroxyethylpiperazine N-2-ethanesulfonic acid as soft templates. Scanning electron microscopic images reveal the bowl-shaped structures surrounded by cauliflower-like fractal PPy films and globular nanostructures. Raman spectroscopy reveals the oxidized nature of the film. Sensing properties of PPy-HRP films for hydrogen peroxide (H2O2) are demonstrated. Electrochemical characterization of the sensor films is done by linear sweep voltammetry (LSV) and amperometry. LSV results indicated the reduction of H2O2 and linearity in response of the sensing film. The amperometric biosensor has a performance comparable to those in the literature with advantages of hard-template free synthesis procedure and a satisfactory sensitivity value of 12.8 mu A/(cm(2) . mM) in the range of 1-10 mM H2O2.

Item Type: Conference Paper
Publication: IEEE Transactions on Instrumentation and Measurement
Publisher: IEEE
Additional Information: Copyright for this article is belongs to IEEE.
Keywords: Electropolymerization;horseradish peroxidase (HRP);hydrogen peroxide biosensor;polypyrrole (PPy) microstructures; zwitterionic surfactant
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 20 Nov 2012 10:24
Last Modified: 20 Nov 2012 10:24
URI: http://eprints.iisc.ac.in/id/eprint/45134

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