Detection of sugar-lectin interactions by multivalent dendritic sugar functionalized single-walled carbon nanotubes

Vasu, KS and Naresh, K and Bagu, RS and Jayaraman, N and Sood, AK (2012) Detection of sugar-lectin interactions by multivalent dendritic sugar functionalized single-walled carbon nanotubes. In: APPLIED PHYSICS LETTERS, 101 (5).

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Abstract

We show that single walled carbon nanotubes (SWNTs) decorated with sugar functionalized poly (propyl ether imine) (PETIM) dendrimer is a very sensitive platform to quantitatively detect carbohydrate recognizing proteins, namely, lectins. The changes in electrical conductivity of SWNT in field effect transistor device due to carbohydrate-protein interactions form the basis of present study. The mannose sugar attached PETIM dendrimers undergo charge-transfer interactions with the SWNTs. The changes in the conductance of the dendritic sugar functionalized SWNT after addition of lectins in varying concentrations were found to follow the Langmuir type isotherm, giving the concanavalin A (Con A)-mannose affinity constant to be 8.5 x 10(6) M-1. The increase in the device conductance observed after adding 10 nM of Con A is same as after adding 20 mu M of a non-specific lectin peanut agglutinin, showing the high specificity of the Con A-mannose interactions. The specificity of sugar-lectin interactions was characterized further by observing significant shifts in Raman modes of the SWNTs. (C) 2012 American Institute of Physics. http://dx.doi.org/10.1063/1.4739793]

Item Type:	Journal Article
Publication:	APPLIED PHYSICS LETTERS
Publisher:	AMER INST PHYSICS
Additional Information:	Copy right for this article belongs to American Ins.Physics, USA
Keywords:	PROTEIN-CARBOHYDRATE INTERACTIONS;MOLECULES;RECOGNITION; MONOLAYERS
Department/Centre:	Division of Chemical Sciences > Organic Chemistry Division of Physical & Mathematical Sciences > Physics
Date Deposited:	15 Oct 2012 05:11
Last Modified:	15 Oct 2012 05:12
URI:	http://eprints.iisc.ac.in/id/eprint/45102

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