Purohit, Madhusudan N and Panjamurthy, Kuppusamy and Elango, Santhini and Hebbar, Karteek and Mayur, Yergeri C and Raghavan, Sathees C (2011) IN-VITRO CYTOTOXICITY AND CELL CYCLE ANALYSIS OF TWO NOVEL BIS-1, 2, 4-TRIAZOLE DERIVATIVES: 1,4-BIS[5-(5-MERCAPTO-1,3,4-OXADIAZOL-2-yl-METHYL)-THIO-4-(p-TOLYL)-1, 2,4-TRIAZOL-3-yl]-BUTANE (MNP-14) AND 1,4-BIS[5-(CARBETHOXY-METHYL)-THIO-4-(p-ETHOXY PHENYL)-1,2,4-TRIAZOL-3-yl]-BUTANE (MNP-16). In: NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 30 (11). pp. 873-885.
Full text not available from this repository. (Request a copy)Abstract
In the present study, we have tested the cytotoxic and DNA damage activity of two novel bis-1,2,4 triazole derivatives, namely 1,4-bis[5-(5-mercapto-1,3,4-oxadiazol-2-yl-methyl)-thio4-(p-tolyl)-1,2 ,4-triazol-3-yl]-butane (MNP-14) and 1,4-bis[5-(carbethoxy-methyl)-thio-4-(p-ethoxy phenyl) -1,2,4-triazol-3-yl]-butane (MNP-16). The effect of these molecules on cellular apoptosis was also determined. The in-vitro cytotoxicity was evaluated by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay as well as Trypan blue dye exclusion methods against human acute lymphoblastic leukemia (MOLT4) and lung cancer cells (A549). Our results showed that MNP-16 induced significant cytotoxicity (IC50 of 3-5 mu M) compared with MNP-14. The cytotoxicity induced by MNP-16 was time and concentration dependent. The cell cycle analysis by flow cytometry (fluorescence-activated cell sorting [FACS]) revealed that though there was a significant increase in the apoptotic population (sub-G1 phase) with an increased concentration of MNP-14 and 16, there was no cell cycle arrest. Further, the comet assay results indicated considerable DNA
| Item Type: | Journal Article |
|---|---|
| Publication: | NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS |
| Publisher: | TaylorA and Francis Inc. |
| Additional Information: | Copyright for this article belongs to Taylor and Francis |
| Keywords: | Cytotoxicity;bis-1, 2, 4-triazole;DNA damage;cell cycle analysis;MTT assay |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 22 May 2012 09:48 |
| Last Modified: | 22 May 2012 09:48 |
| URI: | http://eprints.iisc.ac.in/id/eprint/44197 |
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