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Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy

Mondal, Partha Pratim and Diaspro, Alberto (2011) Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy. In: Scientific Reports, 1 .

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Official URL: http://www.nature.com/srep/2011/111109/srep00149/f...


Fast three-dimensional (3D) imaging requires parallel optical slicing of a specimen with an efficient detection scheme. The generation of multiple localized dot-like excitation structures solves the problem of simultaneous slicing multiple specimen layers, but an efficient detection scheme is necessary. Confocal theta detection (detection at 90 degrees to the optical axis) provides a suitable detection platform that is capable of cross-talk-free fluorescence detection from each nanodot (axial dimension approximate to 150 nm). Additionally, this technique has the unique feature of imaging a specimen at a large working distance with super-resolution capabilities. Polarization studies show distinct field structures for fixed and fluid samples, indicating a non-negligible field-dipole interaction. The realization of the proposed imaging technique will advance and diversify multiphoton fluorescence microscopy for numerous applications in nanobioimaging and optical engineering.

Item Type: Journal Article
Publication: Scientific Reports
Publisher: Nature Publishing Group
Additional Information: Copyright of this article belongs to Nature Publishing Group.
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 16 Mar 2012 11:13
Last Modified: 16 Mar 2012 11:13
URI: http://eprints.iisc.ac.in/id/eprint/44031

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