Investigations on the RNA binding and phosphorylation of groundnut bud necrosis virus nucleocapsid protein

Bhat, Amruta S and Savithri, HS (2011) Investigations on the RNA binding and phosphorylation of groundnut bud necrosis virus nucleocapsid protein. In: Archives of Virology, 156 (12). pp. 2163-2172.

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Abstract

Groundnut bud necrosis virus belongs to the genus Tospovirus, infects a wide range of crop plants and causes severe losses. To understand the role of the nucleocapsid protein in the viral life cycle, the protein was overexpressed in E. coli and purified by Ni-NTA chromatography. The purified N protein was well folded and was predominantly alpha-helical. Deletion analysis revealed that the C-terminal unfolded region of the N protein was involved in RNA binding. Furthermore, the N protein could be phosphorylated in vitro by Nicotiana benthamiana plant sap and by purified recombinant kinases such as protein kinase CK2 and calcium-dependent protein kinase. This is the first report of phoshphorylation of a nucleocapsid protein in the family Bunyaviridae. The possible implications of the present findings for the viral life cycle are discussed.

Item Type:	Journal Article
Publication:	Archives of Virology
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	04 Jan 2012 07:05
Last Modified:	04 Jan 2012 07:05
URI:	http://eprints.iisc.ac.in/id/eprint/42976

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