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Cerebroprotective activity of Wedelia calendulacea on global cerebral ischemia in rats

Prakash, T and Kotresha, D and Rao, Rama N (2011) Cerebroprotective activity of Wedelia calendulacea on global cerebral ischemia in rats. In: Acta Biologica Hungarica, 62 (4). pp. 361-375.

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The present study was to investigate the effect of W. calendulacea on ischemia and reperfusion-induced cerebral injury. Cerebral ischemia was induced by occluding right and left common carotid arteries (global cerebral ischemia) for 30 min followed by reperfusion for 1 h and 4 h individually. Various biochemical alterations, produced subsequent to the application of bilateral carotid artery occlusion (BCAO) followed by reperfusion viz. increase in lipid peroxidation (LPO), hydrogen peroxide (H(2)O(2)), and decrease in reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD), level in the brain tissue, Western blot analysis (Cu-Zn-SOD and CAT) and assessment of cerebral infarct size were measured. All those enzymes are markedly reversed and restored to near normal level in the groups pre-treated with W. calendulacea (250 and 500 mg/kg given orally in single and double dose/day for 10 days) in dose-dependent way. The effect of W. calendulacea had increased significantly the protein expression of copper/zinc superoxide dismutase (Cu-Zn-SOD) and CAT in cerebral ischemia. W. claendulacea was markedly decrease cerebral infarct damages but results are not statistically significant. It can be concluded that W. calendulacea possesses a neuroprotective activity against cerebral ischemia in rat.

Item Type: Journal Article
Publication: Acta Biologica Hungarica
Publisher: Akadémiai Kiadó
Keywords: Wedelia calendulacea;Cu-Zn SOD;cerebral infarction;brain ischemia;Western blot analysis
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 03 Jan 2012 12:31
Last Modified: 03 Jan 2012 12:31
URI: http://eprints.iisc.ac.in/id/eprint/42965

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