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Neuroprotective activity of Wedelia calendulacea on cerebral ischemia/reperfusion induced oxidative stress in rats

Prakash, Tigari and Kotresha, Dupadahalli and Nedendla, Rama Rao (2011) Neuroprotective activity of Wedelia calendulacea on cerebral ischemia/reperfusion induced oxidative stress in rats. In: Indian Journal of Pharmacy, The, 43 (6). pp. 676-682.

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Objective: This study was undertaken to evaluate the neuroprotective activity of Wedelia calendulacea against cerebral ischemia/reperfusion induced oxidative stress in the rats. Materials and Methods: The global cerebral ischemia was induced in male albino Wistar rats by occluding the bilateral carotid arteries for 30 min followed by 1 h and 4 h reperfusion. At various times of reperfusion, the histopathological changes and the levels of malondialdehyde (MDA), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-s-transferase (GST), and hydrogen peroxide (H(2)O(2)) activity and brain water content were measured. Results: The ischemic changes were preceded by increase in concentration of MDA, hydrogen peroxide and followed by decreased GPx, GR, and GST activity. Treatment with W. calendulacea significantly attenuated ischemia-induced oxidative stress. W. calendulacea administration markedly reversed and restored to near normal level in the groups pre-treated with methanolic extract (250 and 500 mg/kg, given orally in single and double dose/day for 10 days) in dose-dependent way. Similarly, W. calendulacea reversed the brain water content in the ischemia reperfusion animals. The neurodegenaration also conformed by the histopathological changes in the cerebral-ischemic animals. Conclusion: The findings from the present investigation reveal that W. calendulacea protects neurons from global cerebral-ischemic injury in rat by attenuating oxidative stress.

Item Type: Journal Article
Publication: Indian Journal of Pharmacy, The
Publisher: Medknow Publications
Additional Information: Copyright of this article belongs to Medknow Publications.
Keywords: Brain edema;global cerebral ischemia;histopathology; oxidative stress;Wedelia calendulacea
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 20 Dec 2011 09:22
Last Modified: 20 Dec 2011 09:22
URI: http://eprints.iisc.ac.in/id/eprint/42778

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