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Design of Affinity Membrane Bioseparations

Sridhar, P (1996) Design of Affinity Membrane Bioseparations. In: Chemical Engineering & Technology, 19 (5). pp. 398-404.


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Affinity separations rely on the highly specific binding between a protein in solution and an immobilized ligand to achieve a high degree of protein purification. A mathematical model including convection, diffusion, and rate kinetics is formulated to analyze the design and operation of affinity membrane bioseparation. The model equations are solved by orthogonal collocation method. Danckwerts' boundary conditions are used. The results obtained from model simulation show that the breakthrough of the protein is significantly influenced by Peclet number, feed protein concentration, Ligand number, Damkohler number, membrane thickness, and flow rate. Breakthrough profiles are quantitatively discussed in terms of protein recovery efficiency, ligand utilization efficiency, thickness of unused membrane, and width of the mass transfer zone.

Item Type: Journal Article
Publication: Chemical Engineering & Technology
Publisher: VCH Verlagsgesellschaft mbH
Additional Information: The copyright belongs to VCH Verlagsgesellschaft mbH.
Department/Centre: Division of Mechanical Sciences > Chemical Engineering
Date Deposited: 28 Nov 2005
Last Modified: 19 Sep 2010 04:21
URI: http://eprints.iisc.ac.in/id/eprint/4069

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