Metallo beta lactamase producing Pseudomonas aeruginosa and Acinetobacter baumannii

John, S and Balagurunathan, R (2011) Metallo beta lactamase producing Pseudomonas aeruginosa and Acinetobacter baumannii. In: Indian Journal of Medical Microbiology, 29 (3). pp. 302-304.

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Abstract

This study aims in identifying MBLs particularly Zn requiring Molecular Class B enzymes produced by Pseudomonas aeruginosa and Acinetobacter baumannii. The resistance by these organisms are in a rise against all antibiotics including carbapenems and no prescribed CLSI guidelines is available for detecting them. Clinical isolates antibiotic susceptibility was determined by number of phenotypic tests by addition of 50mM of 10 mu l zinc as cofactor for metallo beta lactamase production along with 0.5M ETDA of 5 mu l (930 mu g per disk) plain disks. Increase in zone size of the meropenem -EDTA disk compared to the meropenem disk without EDTA was recorded positive. For Zn requiring MBLs zone towards both disks of EDTA and Zn along with meropenem is detected by DDST.

Item Type:	Editorials/Short Communications
Publication:	Indian Journal of Medical Microbiology
Publisher:	Medknow Publications
Additional Information:	Copyright of this article belongs to Medknow Publications.
Keywords:	Metallo-b-lactamases;MIC of meropenem;zinc substitution
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	16 Sep 2011 09:09
Last Modified:	16 Sep 2011 09:09
URI:	http://eprints.iisc.ac.in/id/eprint/40557

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