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ProteoMod: A new tool to quantitate protein post-translational modifications

Kumar, Yadunanda and Khachane, Amit and Belwal, Mukta and Das, Sanjeev and Somsundaram, Kumar and Tatu, Utpal (2004) ProteoMod: A new tool to quantitate protein post-translational modifications. In: Proteomics, 4 (6). pp. 1672-1683.

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Post-translational modifications (PTMs) are known to regulate biological processes by controlling protein function. The effect of a PTM on protein function depends critically on the position and the number of modifications. While there are convenient methods available to qualitatively examine modifications like phosphorylation, glycosylation, acetylation and methylation, methods available for their quantitative assessment are cumbersome. We have developed a new tool that allows quantitation of the number of phosphorylation events in proteins with ease. The ProteoMod tool depends on shifts in the isoelectric points of proteins upon post-translational change. The extent of shift exhibited upon phosphorylation is algorithmically converted into the number of phosphorylations conferred. The validity of ProteoMod was confirmed by examining proteins with previously known number of phosphorylations. The list of proteins examined included HSP27, HSP70 and tumor suppressor p53. The approach can also be applied to estimate modifications like acetylation, methylation and sialylation in proteins. We analyzed shifts in isoelectric points due to sialylation events in N-glycoproteins. Using influenza hemagglutinin we show that shifts in isoelectric points correlate with intracellular distribution of this model membrane protein. In addition to extending the application of two dimensional gel electrophoresis to quantitate modifications, our study also highlights its potential use in cell biology.

Item Type: Journal Article
Publication: Proteomics
Publisher: WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information: The copyright belongs to WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keywords: isoelectric point;phosphorylation;post translational modification;prediction;proteoMod
Department/Centre: Division of Biological Sciences > Biochemistry
Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 17 Nov 2005
Last Modified: 27 Aug 2008 11:33
URI: http://eprints.iisc.ac.in/id/eprint/4035

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