Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity

Sanjay, MK and Srideshikan, SM and Vanishree, VL and Usha, MS and Raj, Philip A and Gaddad, SM and Shivannavar, CT (2011) Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity. In: Indian Journal of Microbiology, 51 (3). pp. 326-331.

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Abstract

Superoxide dismutase has been discovered within the periplasm of several Gram-negative pathogens. We studied the Cu,Zn-SOD enzyme in Escherichia coli isolated from clinical samples (stool samples) collected from patients suffering from diarrhea. Antibiogram studies of the isolates were carried out to determine the sensitive and resistant strains. The metal co-factor present in the enzyme was confirmed by running samples in native gels and inhibiting with 2 mM potassium cyanide. A 519 bp sodC gene was amplified from resistant and sensitive strains of Escherichia coli. Cloning and sequencing of the sodC gene indicated variation in the protein and amino acid sequences of sensitive and resistant isolates. The presence of sodC in highly resistant Escherichia coli isolates from diarrheal patients indicates that sodC may play role in enhancing the pathogenicity by protecting cells from exogenous sources of superoxide, such as the oxidative burst of phagocytes. The presence of SodC could be one of the factors for bacterial virulence.

Item Type:	Journal Article
Publication:	Indian Journal of Microbiology
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Gram-negative bacteria;Escherichia coli;Drug resistance; Superoxide dismutase;sodC;Pathogenicity
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	01 Aug 2011 07:22
Last Modified:	01 Aug 2011 07:22
URI:	http://eprints.iisc.ac.in/id/eprint/39605

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