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Role of TGF-beta beta and BMP7 in the pathogenesis of oral submucous fibrosis

Khan, Imran and Agarwal, Prasoon and Thangjam, Gagan Singh and Radhesh, Rekha and Rao, Girish S and Kondaiah, Paturu (2011) Role of TGF-beta beta and BMP7 in the pathogenesis of oral submucous fibrosis. In: Growth Factors, 29 (4). pp. 119-127.

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Official URL: http://www.ingentaconnect.com/content/apl/ggrf/201...


To understand the molecular pathogenesis of oral submucous fibrosis (OSF), which is a chronic inflammatory disease, gene expression profiling was performed in 10 OSF tissues against 8 pooled normal tissues using oligonucleotide arrays. Microarray results revealed differential expression of 5288 genes (P < a parts per thousand currency sign 0.05 and fold change >= a parts per thousand yen 1.5). Among these, 2884 are upregulated and 2404 are downregulated. Validation employing quantitative real-time PCR and immunohistochemistry confirmed upregulation of transforming growth factor-beta beta 1 (TGF-beta beta 1), TGFBIp, THBS1, SPP1, and TIG1 and downregulation of bone morphogenic protein 7 (BMP7) in OSF tissues. Furthermore, activation of TGF-beta beta pathway was evident in OSF as demonstrated by pSMAD2 strong immunoreactivity. Treatment of keratinocytes and oral fibroblasts by TGF-beta beta confirmed the regulation of few genes identified in microarray including upregulation of connective tissue growth factor, TGM2, THBS1, and downregulation of BMP7, which is a known negative modulator of fibrosis. Taken together, these data suggest activation of TGF-beta beta signaling and suppression of BMP7 expression in the manifestation of OSF.</.

Item Type: Journal Article
Publication: Growth Factors
Publisher: Informa plc
Additional Information: Copyright of this article belongs to Informa plc.
Keywords: Arecoline;inflammation;epithelial-stromal interaction
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 03 Aug 2011 05:49
Last Modified: 03 Aug 2011 05:49
URI: http://eprints.iisc.ac.in/id/eprint/39596

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