Syed, AA and Amshumali, MK and Devan, N (2002) Chromatographic methods for determination of nimesulide and for stability studies. In: Acta Chromatographica, 12 . pp. 95-103.
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Simple and rapid HPLC, GC, and TLC procedures have been developed for detection and determination of nimesulide, a non-pharmacopeial drug, in preformulation and dosage form. Use of these techniques has enabled separation of impurities and the precursor in the bulk material and in formulations. Isocratic reversed-phase HPLC was performed on a C-18 column with methanol-water-acetic acid, 67:32:1 (v/v), as mobile phase and UV detection at 230 nm. Calibration curves were linear over the concentration range 100-1000 mug mL(-1) with a good correlation coefficient (0.9993) and a coefficient of variation of 1.5%. Gas chromatography was performed on an OV-17 packed column with temperature programming and flame-ionization detection. The lower limit of determination by HPLC and GC was 4 ppm. Thin-layer chromatography of nimesulide was performed on silica gel G with toluene-ethyl acetate, 8:2, as mobile phase. Stability testing of the drug was performed under different temperature, humidity, and UV-radiation conditions.
| Item Type: | Journal Article |
|---|---|
| Publication: | Acta Chromatographica |
| Publisher: | Akadémiai Kiadó |
| Additional Information: | Copyright of this article belongs to Akadémiai Kiadó. |
| Department/Centre: | Division of Chemical Sciences > Organic Chemistry |
| Date Deposited: | 22 Jul 2011 09:14 |
| Last Modified: | 22 Jul 2011 09:14 |
| URI: | http://eprints.iisc.ac.in/id/eprint/39050 |
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