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The genetics of Mycobacterium tuberculosis

Vijaya, S (1998) The genetics of Mycobacterium tuberculosis. In: Journal of Genetics, 77 (2-3). pp. 123-128.

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Gene manipulation in Mycobacterium tuberculosis has been slow in coming of age owing to the inherent difficulties associated with working on this aerosol-transmitted pathogen, in addition to the paucity of molecular tools such as plasmids and transposons. One of the early approaches to overcome these difficulties was the development of phasmids, which combined the properties of phages and plasmids and allowed introduction of recombinant genes into mycobacteria. The lone plasmid pAL5000 of mycobacteria has been exploited to its fullest potential in the construction of a plethora of vectors. Above all, the single most important achievement has been the development of elegant and innovative approaches to overcome the problem of illegitimate recombination which threatened the success of allelic-exchange mutagenesis in the slow-growing pathogenic mycobacterial species. In this review I discuss the current status of conditionally replicating plasmid and transposon vectors and their application in generating targeted mutations in mycobacteria.

Item Type: Journal Article
Publication: Journal of Genetics
Publisher: Indian Academy of Sciences
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Keywords: Mycobacterium tuberculosis;molecular genetics;transposons; temperate mycobacteriophages;phasmids;gene disruption.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 12 Jul 2011 07:49
Last Modified: 12 Jul 2011 07:49
URI: http://eprints.iisc.ac.in/id/eprint/38784

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