Preincubation of cut tobacco leaf explants promotes Agrobacterium-mediated transformation by increasing vir gene induction

Sunilkumar, G and Vijayachandra, K and Veluthambi, K (1999) Preincubation of cut tobacco leaf explants promotes Agrobacterium-mediated transformation by increasing vir gene induction. In: Plant Science, 141 (1). 51-58 .

PDF Preincubation_of_cut_tobacco.pdf - Published Version Restricted to Registered users only Download (304kB) | Request a copy

Abstract

The effects of preincubation of cut tobacco leaf explants on Agrobacterium transformation efficiency and induction of Agrobacterium virE-lacZ fusion were evaluated. Transformation efficiency was evaluated by histochemical and fluorometric analysis of beta-glucuronidase in leaf rings transformed with Agrobacterium tumefaciens strain LBA4404(pKIWI105). The transformation efficiency increased by 2-fold, 5-fold, and 4.3-fold upon preincubation for 24, 48, and 72 h, respectively. Preincubation for 24, 48, and 72 h increased the ability of tobacco leaf segments to induce Agrobacterium virE by 2.3-fold, 3.5-fold and 4.5-fold, respectively. The requirement of preincubation for increased transformation efficiency was obviated by the addition of 100 mu M acetosyringone to the freshly cut leaf rings cocultivated with Agrobacterium. The production of vii gene inducers by the leaf rings during the preincubation period is an important factor that contributes to increased transformation efficiency of Agrobacterium upon preincubation. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

Item Type:	Journal Article
Publication:	Plant Science
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Acetosyringone;Agrobacterium;Preincubation;Transformation efficiency;6ir induction;Wounding
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	28 Jun 2011 09:23
Last Modified:	02 Nov 2011 08:02
URI:	http://eprints.iisc.ac.in/id/eprint/38731

Actions (login required)

View Item