Venkatachalam, P and Geetha, N and Jayabalan, N (1997) In vitro regeneration from immature cotyledon explant and protein profile changes during organogenesis in groundnut (Arachis hypogaea L.). In: Tropical Agriculture, 74 (2). pp. 140-145.
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Complete plants were regenerated from in vitro cultured immature cotyledon segments of groundnut (Arachis hypogaea L. cv. TMV-7) by organogenesis. Callus cultures were best Initiated from immature cotyledon segments on MS (Murashige and Skoog) salts containing B5 vitamins supplemented with indole-3-acetic acid (IAA) and alpha -naphthalene acetic acid (NAA; 4.0 mg L-1) and kinetin (KIN; 0.5 L-1). Calluses were transferred to a medium containing KIN (2.0 mg L-1) and IAA and NAA (0.5 mg L-1) for shoot Initiation. The regenerated shoots were transferred to a medium containing Indole-3-butyric acid (IBA; 2.0 mg L-1) and KIN (0.2 mg L-1) for developing roots. In vitro produced plantlets developed sucessfully, matured, and set seed. The protein profiles [sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE)] of callus, callus with shoot, and callus with shoot and root showed differences.
| Item Type: | Journal Article |
|---|---|
| Publication: | Tropical Agriculture |
| Publisher: | TROPICAL AGRICULTURE |
| Additional Information: | Copyright of this article belongs to TROPICAL AGRICULTURE. |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 18 Oct 2011 04:37 |
| Last Modified: | 18 Oct 2011 04:37 |
| URI: | http://eprints.iisc.ac.in/id/eprint/38277 |
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