Sensitivity Enhancement in Solid-State Separated Local Field NMR Experiments by the Use of Adiabatic Cross-Polarization

Lobo, Nitin P and Ramanathan, Krishna V (2011) Sensitivity Enhancement in Solid-State Separated Local Field NMR Experiments by the Use of Adiabatic Cross-Polarization. In: The Journal of Physical Chemistry Letters, 2 (10). pp. 1183-1188.

PDF Sensitivity_Enhancement.pdf - Published Version Restricted to Registered users only Download (3MB) | Request a copy

Abstract

Measurement of dipolar couplings using separated local field (SLF) NMR experiment is a powerful tool for structural and dynamics studies of oriented molecules such as liquid crystals and membrane proteins in aligned lipid bilayers. Enhancing the sensitivity of such SLF techniques is of significant importance in present-day solid-state NMR methodology. The present study considers the use of adiabatic cross-polarization for this purpose, which is applied for the first time to one of the well-known SLF techniques, namely, polarization inversion spin exchange at the magic angle (PISEMA). The experiments have been carried out on a single crystal of a model peptide, and a dramatic enhancement in signal-to-noise up to 90% has been demonstrated.

Item Type:	Journal Article
Publication:	The Journal of Physical Chemistry Letters
Publisher:	American Chemical Society
Additional Information:	Copyright of this article belongs to American Chemical Society.
Department/Centre:	Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility) Division of Physical & Mathematical Sciences > Physics
Date Deposited:	17 Jun 2011 07:56
Last Modified:	17 Jun 2011 07:56
URI:	http://eprints.iisc.ac.in/id/eprint/38221

Actions (login required)

View Item