Production of biosurfactant “Biosur-Pm” by Pseudomonas maltophila CSV89: characterization and role in hydrocarbon uptake

Phale, Prashant S and Savithri, Handanahal Subbarao and Rao, Appaji N and Vaidyanathan, Chelakara S (1995) Production of biosurfactant “Biosur-Pm” by Pseudomonas maltophila CSV89: characterization and role in hydrocarbon uptake. In: Archives of Microbiology, 163 (6). pp. 424-431.

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Abstract

Pseudomonas maltophilia CSV89, a soil bacterium, produces an extracellular biosurfactant, ''Biosur-Pm''. The partially purified product is nondialyzable and chemically composed of 50% protein and 12-15% sugar, which indicates the complex nature of Biosur-Pm. It reduces the surface tension of water from 73 to 53 x 10(-3) N m(-1) and has a critical micellar concentration of 80 mg/l. Compared to aliphatic hydrocarbons, Biosur-Pm shows good activity against aromatic hydrocarbons. The emulsion formed is stable and does not require any metal ions for emulsification. The kinetics of Biosur-Pm production suggest that its synthesis isa growth-associated and pH-dependent process. At pH 7.0, cells produced more Biosur-Pm with less cell surface hydrophobicity. At pH 8.0, however, the cells produced less Biosur-Pm with more cell surface hydrophobicity and showed a twofold higher affinity for aromatic hydrocarbons compared to the cells grown at pH 7.0. The Biosur-Pm showed a pH-dependent release, stimulated growth of the producer strain on mineral salts medium with 1-naphthoic acid when added externally, and facilitated the conversion of salicylate to catechol. All these results suggest that Biosur-Pm is probably a cell-wall component and helps in hydrocarbon assimilation/uptake.

Item Type:	Journal Article
Publication:	Archives of Microbiology
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Biosurfactants;Pseudomonas;Hydrocarbon degradation; Hydrocarbon assimilation/uptake
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	02 Jun 2011 10:27
Last Modified:	02 Jun 2011 10:27
URI:	http://eprints.iisc.ac.in/id/eprint/38155

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