DNA recognition by the EcoP15I and EcoPI modification methyltransferases

Ahmad, Ishtiyaque and Krishnamurthy, Vinita and Rao, Desirazu N (1995) DNA recognition by the EcoP15I and EcoPI modification methyltransferases. In: Gene, 157 (1-2). pp. 143-147.

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Abstract

The DNA-binding properties of the EcoP15I DNA methyltransferase (M . EcoP15I; MTase) were studied using electrophoretic mobility shift assays. We show by molecular size-exclusion chromatography and dimethyl suberimidate crosslinking that M . EcoP15I is a dimer in solution. While M . EcoP15I binds approx. threefold more tightly to its recognition sequence, 5'-CAGCAG-3', than to non-specific sequences in the presence of AdoMet or its analogs, the discrimination between specific and non-specific sequences significantly increases in presence of ATP. These results suggest for the first time a role for ATP in DNA recognition by type-III restriction-modification enzymes. Furthermore, we show that although c2 EcoPI mutant MTases are defective in AdoMet binding, they are still able to bind DNA in a sequence-specific manner.

Item Type:	Journal Article
Publication:	Gene
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	31 May 2011 08:58
Last Modified:	31 May 2011 08:58
URI:	http://eprints.iisc.ac.in/id/eprint/38007

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