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A novel zinc bis(thiosemicarbazone) complex for live cell imaging

Dayal, Disha and Palanimuthu, Duraippandi and Shinde, Sridevi Vijay and Somasundaram, Kumaravel and Samuelson, Ashoka G (2011) A novel zinc bis(thiosemicarbazone) complex for live cell imaging. In: Journal of Biological Inorganic Chemistry, 16 (4). pp. 621-632.

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Fluorescent zinc complexes have recently attracted a lot of interest owing to their vast applications in cellular imaging. We report the synthesis as well as physical, chemical and biological studies of a novel zinc glyoxalbis(4-methyl-4-phenyl-3-thiosemicarbazone), Zn (GTSC)](3), complex. As compared with the well-studied zinc biacetylbis(4-methyl-3-thiosemicarbazone), Zn(ATSM), complex, which was used as a reference, Zn(GTSC)](3) had 2.5-fold higher fluorescence. When cellular fluorescence was measured using flow cytometry, we observed that Zn(GTSC)](3) had 3.4-fold to 12-fold higher fluorescence than Zn(ATSM) in various cell lines (n = 9) of different tissue origin. Confocal fluorescence microscopy results showed that Zn(GTSC)](3) appeared to have a nuclear localization within 30 mm of addition to MCF7 cells. Moreover, Zn(GTSC)](3) showed minimal cytotoxicity compared with Zn(ATSM), suggesting that Zn(GTSC)](3) may be less deleterious to cells when used as an imaging agent. Our data suggest that the novel Zn(GTSC)](3) complex can potentially serve as a biocompatible fluorescent imaging agent for live cells.

Item Type: Journal Article
Publication: Journal of Biological Inorganic Chemistry
Publisher: Springer
Additional Information: Copyright of this article belongs to Springer.
Keywords: Bis(thiosemicarbazone);Imaging agent;Cell viability;Breast cancer;Zinc
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited: 01 Jun 2011 11:52
Last Modified: 01 Jun 2011 11:52
URI: http://eprints.iisc.ac.in/id/eprint/37807

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