Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress

Muthukumar, Kannan and Rajakumar, Selvaraj and Sarkar, Mary Nirmala and Nachiappan, Vasanthi (2011) Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress. In: Antonie van Leeuwenhoek, 99 (4). pp. 761-771.

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Abstract

The present study was undertaken to determine the role of glutathione peroxidase3 (gpx3) in phospholipid protection in cells. Wild-type (WT) cells showed an overall increase in phospholipids upon 50 mu M cadmium (Cd)-treatment, whereas an untreated gpx3 Delta strain showed a drastic reduction in overall phospholipids which was further reduced with 50 mu M Cd. In WT cells, Cd-exposure increased the short chain fatty acids and decreased the unsaturated fatty acids and the magnitude was high in Cd-treated gpx3 Delta cells. Purified recombinant gpx3p showed higher activity with phospholipid hydroperoxides than shorter hydroperoxides. An increase in gpx activity was observed in Cd-treated WT cells and no such alteration was observed in gpx3 Delta. WT cells treated with Cd showed an increase in MDA over untreated, while untreated gpx3 Delta cells themselves showed a higher level of MDA which was further enhanced with Cd-treatment. Iron, zinc and calcium levels were significantly altered in WT and gpx3 Delta cells during Cd-treatment.

Item Type:	Journal Article
Publication:	Antonie van Leeuwenhoek
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Glutathione peroxidase;Phospholipids;Fatty acids; Antioxidants;Cadmium;Saccharomyces cerevisiae and oxidative stress
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	20 May 2011 07:18
Last Modified:	20 May 2011 07:18
URI:	http://eprints.iisc.ac.in/id/eprint/37692

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