Effect of beta-lactamase-catalyzed hydrolysis of cephalosporins on peroxynitrite-mediated nitration of serum albumin and cytochrome c

Jakka, Surendar Reddy and Mugesh, Govindasamy (2011) Effect of beta-lactamase-catalyzed hydrolysis of cephalosporins on peroxynitrite-mediated nitration of serum albumin and cytochrome c. In: Indian Journal of Chemistry - Section A: Inorganic, Physical, Theoretical and Analytical Chemistry, 50 (3-4, S). pp. 447-452.

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Abstract

The hydrolysis of beta-lactam antibiotics by beta-lactamases is one of the major bacterial defense systems. These enzymes generally hydrolyze a variety of antibiotics including the latest generation of cephalosporins, cephamycins and imipenem. In this paper, the effect of cephalosporins-based antibiotics on the peroxynitrite-mediated nitration of protein tyrosine is described. Although some of the antibiotics have weak inhibitory effect on the nitration reactions in the absence of beta-lactamase, they exhibit very strong inhibition in the presence of beta-lactamase. This is due to the elimination of heterocyclic thiol/thione moieties from cephalosporins by beta-lactamase-mediated hydrolysis. After the elimination, the thiols/thiones effectively scavenge peroxynitrite, leading to the inhibition of the nitration reactions.

Item Type:	Journal Article
Publication:	Indian Journal of Chemistry - Section A: Inorganic, Physical, Theoretical and Analytical Chemistry
Publisher:	National Institute of Science Communication and Information Resources
Additional Information:	Copyright of this article belongs to National Institute of Science Communication and Information Resources.
Keywords:	Bioinorganic chemistry;Hydrolysis;Enzyme hydrolysis; Nitration;Protein nitration;Cephalosporins;Tyrosine;Lactams
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	20 May 2011 06:58
Last Modified:	20 May 2011 06:58
URI:	http://eprints.iisc.ac.in/id/eprint/37684

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