Development of specific DNA probes and their usage in the detection of Plasmodium vivax infection in blood

Ayyanathan, K and Francis, VSNK and Datta, S and Padmanaban, G (1995) Development of specific DNA probes and their usage in the detection of Plasmodium vivax infection in blood. In: Molecular and Cellular Probes, 9 (4). pp. 239-246.

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Abstract

The application of nucleic acid probes, in the detection of pathogenic micro-organisms, has become an integral part of diagnostic technologies. In this study, Plasmodium vivax-specific DNA probes have been identified by carrying out genomic subtractive hybridization. In this approach, the recombinant clones from a P. vivax genomic library are screened with radiolabelled human and P. falciparum DNA. The colonies which react with labelled P. falciparum and human DNA are eliminated and those which do not produce any autoradiographic signal have been subjected to further screening procedures. Three Fl vivax specific DNA probes have been obtained by these repeated screenings. Further analyses indicate that these probes are specific and sensitive enough to detect P. vivax infection in clinical blood samples when used in a non-radioactive DNA hybridization assay. (C) 1995 Academic Press Limited

Item Type:	Journal Article
Publication:	Molecular and Cellular Probes
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Plasmodium vivax;DNA probes;genomic subtractive hybridization;non-radioactive DNA diagnostics
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	15 Jun 2011 08:46
Last Modified:	15 Jun 2011 08:46
URI:	http://eprints.iisc.ac.in/id/eprint/37545

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