Realtime kinetic analysis of antigen-antibody interaction using solid phase binding: Transformation of hCG-monoclonal antibody complex

Murthy, GS (1996) Realtime kinetic analysis of antigen-antibody interaction using solid phase binding: Transformation of hCG-monoclonal antibody complex. In: Current Science (Bangalore), 71 (12). pp. 981-988.

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Abstract

Kinetic constants of MAb-hCG interactions have been determined using solid phase binding of I-125[hCG] to immobilized MAb. While association has been shown to follow the expected pattern, dissociation consists of at least two reversible steps, one with a rate constant of 0.0025 min(-1), and a second with a rate constant of 0.00023 min(-1). Validity of affinity constant measurements in the light of the complex reaction kinetics is discussed, A comparison between the method of surface plasmon resonance technology (BIAcore) and solid phase binding (SPB) for determination of kinetic parameters shows that SPB provides not only a cost-effective approach for determination of realtime kinetic parameters of macromolecular ligand-ligate interaction but also a method with several advantages over the BIAcore system in investigating the mechanism of antigen-antibody interaction.

Item Type:	Journal Article
Publication:	Current Science (Bangalore)
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Biological Sciences > Central Animal Facility (Formerly Primate Research Laboratory)
Date Deposited:	10 May 2011 07:53
Last Modified:	21 Oct 2011 06:23
URI:	http://eprints.iisc.ac.in/id/eprint/37507

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