Interactions of 3' terminal and 5' terminal regions of physalis mottle virus genomic RNA with its replication complex

Srividhya, V and Savithri, HS (1996) Interactions of 3' terminal and 5' terminal regions of physalis mottle virus genomic RNA with its replication complex. In: Journal of Biosciences, 21 (6). pp. 743-753.

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Abstract

Physalis mottle virus (PhMV) belongs to the tymogroup of positive-strand RNA viruses with a genome size of 6 kb. Crude membrane preparations from PhMV-infected Nicotiana glutinosa plants catalyzed the synthesis of PhMV genomic RNA from endogenously bound template. Addition of exogenous genomic RNA enhanced the synthesis which was specifically inhibited by the addition of sense and antisense transcripts corresponding to 3' terminal 242 nucleotides as well as the 5' terminal 458 nucleotides of PhMV genomic RNA while yeast tRNA or ribosomal RNA failed to inhibit the synthesis. This specific inhibition suggested that the 5' and 3' non-coding regions of PhMV RNA might play an important role in viral replication.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian academy of sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	Physalis mottle virus;replication;inhibition;SSRNA plant virus.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	29 Apr 2011 06:45
Last Modified:	29 Apr 2011 06:45
URI:	http://eprints.iisc.ac.in/id/eprint/37323

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