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Cloning And Characterization of Mycobacteriophage-13 Promoters

Ramesh, GR and Gopinathan, KP (1995) Cloning And Characterization of Mycobacteriophage-13 Promoters. In: Indian Journal of Biochemistry & Biophysics, 32 (6). pp. 361-367.

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Restriction fragments of mycobacteriophage 13 DNA capable of initiating transcription have been cloned into a promoter selection vector of Escherichia coli, and selected on the basis of development of resistance to chloramphenicol. The growth pattern of these 'promoter clones' on a concentration gradient of chloramphenicol and the biochemical assays of the chloramphenicol acetyl transferase have permitted the assessment of their relative promoter strengths. DNA sequence analysis revealed significant homology of these promoters to the -35 regions of the mycobacterial- and E. coli promoter consensus, but less so to the - 10 region. Based on the sequence of phage 13 promoters identified here and the reported sequences of mycobacterial promoters, a promoter consensus for mycobacteria has been generated.

Item Type: Journal Article
Publication: Indian Journal of Biochemistry & Biophysics
Publisher: National Institute of Science Communication and Information Resources
Additional Information: Copyright of this article belongs to National Institute of Science Communication and Information Resources.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 15 Jun 2011 09:30
Last Modified: 25 Oct 2018 10:34
URI: http://eprints.iisc.ac.in/id/eprint/37237

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