Subramanian, S and Karande, AA and Adiga, PR (1996) Establishment of the functional importance of thiamin carrier protein in pregnant rats by using monoclonal antibodies. In: Indian Journal of Biochemistry & Biophysics, 33 (2). pp. 111-115.
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Monoclonal antibodies (mAbs) to chicken thiamin carrier protein (TCP) have been produced by hybridoma technology to identify the crucial epitopes involved in bioneutralization of the vitamin carrier. The monoclonality of these mAbs (A4C4, F3H6, H8H3, C8C1 and G7H10) was sought to be confirmed by sub-class isotyping; they all belong to IgG1, k type. The epitopes recognized by all the five mAbs are conserved in TCP from the chicken to the rat as assessed by liquid phase RIA and immunoprecipitation of I-125-labelled proteins from pregnant rat serum. Among these mAbs, passive immunization of pregnant rats with the mAb C8C1 only on three consecutive days (day 10, 11 and 12) resulted in embryonic resorption. These results demonstrate the importance of epitopic structure specified by the mAb C8C1 on TCP during pregnancy in rats.
| Item Type: | Journal Article |
|---|---|
| Publication: | Indian Journal of Biochemistry & Biophysics |
| Publisher: | National institute of science communication and information resources |
| Additional Information: | Copyright of this article belongs to National institute of science communication and information resources. |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
| Date Deposited: | 26 Apr 2011 06:38 |
| Last Modified: | 23 Oct 2018 10:08 |
| URI: | http://eprints.iisc.ac.in/id/eprint/37169 |
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