Dissociation of monoclonal antibody-antigen complexes: Implications for ELISA procedures

Venkatesh, N and Murthy, GS (1996) Dissociation of monoclonal antibody-antigen complexes: Implications for ELISA procedures. In: Journal of Immunological Methods, 199 (2). pp. 167-174.

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Abstract

A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic,gonadotropin (hCG) using monoclonal antibodies (MAb) from culture media adsorbed immunochemically on plastic tubes. The assays have been found to be very simple in terms of operation and do not demand purification of MAbs. Several MAbs which do not show any displacement in liquid phase RIA and ELISA provide a satisfactory SS-SPRIA. Our investigations revealed that the assumption regarding the stability of the primary Mab-Ag complex during incubation and washing steps in ELISAs is not strictly valid for dissociable MAbs. A comparison of different assay systems suggests that the single step SPRIA offers additional advantages over conventionally used multistep ELISA procedures and provides a quantitative probe for the analysis of epitope-paratope interactions.

Item Type:	Journal Article
Publication:	Journal of Immunological Methods
Publisher:	Elsevier science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Monoclonal antibody;Human chorionic gonadotropin;Solid phase radioimmunoassay:ELISA;Epitope stability:Monoclonal antibody-human chorionic gonadotropin dissociation
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	05 May 2011 04:59
Last Modified:	08 Jul 2011 08:11
URI:	http://eprints.iisc.ac.in/id/eprint/37114

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