Azidothymidine induces apoptosis in mouse myeloma cell line Sp2/0

Sailaja, G and Nayak, R and Antony, A (1996) Azidothymidine induces apoptosis in mouse myeloma cell line Sp2/0. In: Biochemical Pharmacology, 52 (6). pp. 857-862.

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Abstract

Azidothymidine (AZT), which has been extensively used as an antiviral agent in the treatment of AIDS, showed strong inhibition of growth of Sp2/0 cells in vitro. AZT-treated cells showed a decrease in viability in a dose-dependent manner. AZT specifically induced typical apoptotic cell death with DNA double-strand cleavage and subsequent formation of apoptotic bodies. The induction of DNA double-strand cleavage into the oligonucleosomal ladder by AZT was protected in the presence of thymidine or uridine. An increase in endonuclease activity from nuclear extract of AZT-treated cells was observed. The enzyme activity was found to be Ca2+- and Mg2+-dependent and was inhibited by zinc acetate. A marked enhancement of PARP activity was observed in AZT-treated cells. These observations show that AZT can trigger both morphological and biochemical changes typical of apoptosis in the mouse myeloma cell line Sp2/0.

Item Type:	Journal Article
Publication:	Biochemical Pharmacology
Publisher:	Elsevier science
Additional Information:	Copyright of this article belongs to Elsevier science.
Keywords:	azidothymidine;apoptosis;endonuclease;poly(ADP-ribose) polymerase
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	21 Apr 2011 05:46
Last Modified:	21 Apr 2011 05:46
URI:	http://eprints.iisc.ac.in/id/eprint/37045

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