Immune responses to hemagglutinin-neuraminidase protein of peste des petits ruminants virus expressed in transgenic peanut plants in sheep

Khandelwal, Abha and Renukaradhya, Gourapura J and Rajasekhar, Malleshappa and Sita, Lakshmi G and Shaila, Melkote S (2011) Immune responses to hemagglutinin-neuraminidase protein of peste des petits ruminants virus expressed in transgenic peanut plants in sheep. In: Veterinary Immunology and Immunopathology, 140 (3-4). pp. 291-296.

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Abstract

Peste des petits ruminants (PPR) is an acute, highly contagious disease of small ruminants caused by a morbillivirus, Peste des petits ruminants virus (PPRV). The disease is prevalent in equatorial Africa, the Middle East, and the Indian subcontinent. A live attenuated vaccine is in use in some of the countries and has been shown to provide protection for at least three years against PPR. However, the live attenuated vaccine is not robust in terms of thermotolerance. As a step towards development of a heat stable subunit vaccine, we have expressed a hemagglutinin-neuraminidase (HN) protein of PPRV in peanut plants (Arachis hypogea) in a biologically active form, possessing neuraminidase activity. Importantly. HN protein expressed in peanut plants retained its immunodominant epitopes in their natural conformation. The immunogenicity of the plant derived HN protein was analyzed in sheep upon oral immunization. Virus neutralizing antibody responses were elicited upon oral immunization of sheep in the absence of any mucosal adjuvant. In addition, anti-PPRV-HN specific cell-mediated immune responses were also detected in mucosally immunized sheep. (C) 2010 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	Veterinary Immunology and Immunopathology
Publisher:	Elsevier Science B.V.
Additional Information:	Copyright of this article belongs to Elsevier Science B.V.
Keywords:	Peste des petits ruminants virus;Hemagglutinin-neuraminidase;Transgenic peanut;Oral immunogenicity
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	19 Apr 2011 06:02
Last Modified:	19 Apr 2011 06:02
URI:	http://eprints.iisc.ac.in/id/eprint/36940

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