A pathway for biodegradation of 1-naphthoic acid by Pseudomonas maltophilia CSV89

Phale, Prashant S and Mahajan , Milind C (1995) A pathway for biodegradation of 1-naphthoic acid by Pseudomonas maltophilia CSV89. In: Archives of Microbiology, 163 (1). pp. 42-47.

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Abstract

Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates.

Item Type:	Journal Article
Publication:	Archives of Microbiology
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Pseudomonas;1-Naphthoic acid;Hydrocarbon degradation; Hydroxylation;Ring cleavage
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	26 Apr 2011 06:19
Last Modified:	26 Apr 2011 06:19
URI:	http://eprints.iisc.ac.in/id/eprint/36920

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