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Recombinant mid gut antigen (Bm95) as a vaccine against Indian Rhiphicephalus haemaphysaloides in Bos indicus cattle

Sugumar, Parthasarathy and Chandran, Dev and Rani, Gudavalli Sudha and Shahana, Pallichera Vijayan and Maske, Dasarath Keshavrao and Rangarajan, Pundi Narasimhan and Mangamoori, Lakshmi Narasu and Srinivasan, Villuppanoor Alwar (2011) Recombinant mid gut antigen (Bm95) as a vaccine against Indian Rhiphicephalus haemaphysaloides in Bos indicus cattle. In: Research in Veterinary Science, 90 (2). pp. 262-268.

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Official URL: http://dx.doi.org/10.1016/j.rvsc.2010.05.037


In the present study, we report for the first time the efficacy of recombinant Bm95 mid gut antigen isolated from an Argentinean strain of Rhipicephalus microplus strain A in controlling the tick infestations in India. The synthetic gene for Bm95 optimized for expression in yeast was obtained and used to generate yeast transformants expressing Bm95 which was purified to apparent homogeneity. Liquid chromatography-mass spectrometry analysis of the purified protein confirmed its identity as Bm95. Vaccine was prepared by blending various concentrations of purified Bm95 with aluminium hydroxide as an adjuvant. lmmunogenicity studies of the vaccine in rabbits and cattle indicated that the vaccine was highly immunogenic. The efficacy studies of the vaccine was done in cattle. Naive Bos indicus cattle were vaccinated with the recombinant vaccine and were challenged with the larval, nymphal and adult forms of Rhiphicephalus haemaphysaloides. The vaccine protected the animals from larval, nymph and adult tick challenges with an efficacy of 98.7%, 84.6% and 78.9% respectively. The results obtained from the above studies clearly demonstrated the advantage and possibilities of the use of Bm95 in controlling R. haemaphysaloides infestations in the field. (C) 2010 Elsevier Ltd. All rights reserved.

Item Type: Journal Article
Publication: Research in Veterinary Science
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Tick;Bm95;Rhiphicephalus;Tick-borne diseases
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 20 Apr 2011 05:20
Last Modified: 20 Apr 2011 05:20
URI: http://eprints.iisc.ac.in/id/eprint/36723

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