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High level stable expression of rat brain type IIA sodium channel ?-subunit in CHO cells

Sarkar, SN and Sikdar , SK (1994) High level stable expression of rat brain type IIA sodium channel ?-subunit in CHO cells. In: Current science (Bangalore), 67 (3). 196 -199 .

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The neuronal sodium channels are responsible for the rising phase of action potential and are composed of three subunits, of which the alpha-subunit has been shown to be adequate for most of its functional properties. We have stably expressed the rat brain type IIA sodium channel alpha-subunit in CHO cell tine using a CMV promoter-based vector. The expression was confirmed by detecting a 6.5 kb RNA corresponding to sodium channel alpha-subunit using Northern hybridization. The cells stably expressing the alpha-subunit, yield isolated sodium currents of amplitudes greater than 4nA when studied in whole-cell configuration of the patch-clamp technique. The sodium currents are characterized by activation and inactivation properties similar to neuronal sodium channels, and are blocked by the voltage gated sodium channel blocker tetrodotoxin (TTX).

Item Type: Editorials/Short Communications
Publication: Current science (Bangalore)
Publisher: Indian academy of sciences
Additional Information: Copyright of this article belongs to Indian academy of sciences.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 01 Apr 2011 05:14
Last Modified: 01 Apr 2011 05:14
URI: http://eprints.iisc.ac.in/id/eprint/36532

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