Cloning of a buffalo (Bubalus bubalis) prostaglandin $F_2_\alpha$ receptor: changes in its expression and concentration in the buffalo cow corpus luteum

Kumar, Shalu Verma and Srinivas, SV and Muraly, P and Yadav, Vijay Kumar and Medhamurthy, R (2004) Cloning of a buffalo (Bubalus bubalis) prostaglandin $F_2_\alpha$ receptor: changes in its expression and concentration in the buffalo cow corpus luteum. In: Reproduction, 127 . pp. 705-715.

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Abstract

Acting primarily through its specific G protein-coupled receptor termed FPr, prostaglandin (PG) $F_2_\alpha)$ induces regression of the corpus luteum (CL) at the end of a non-fertile oestrous cycle. This study was aimed at cloning a full-length cDNA for FPr and determining its expression and protein concentrations during different stages of CL development in the water buffalo. Serum progesterone and StAR expression were determined to establish temporal relationships between indices of steroidogenesis and changes in FPr expression at different stages of CL development. In contrast to the dairy cow, the stage IV CL (day 20 of the oestrous cycle) did not appear to be functionally regressed in the buffalo. Molecular cloning of a cDNA encoding the buffalo FPr yielded a full length 2193 bp FPr cDNA containing a single open reading frame encoding a 362 amino acid protein with seven putative membrane-spanning domains. The deduced buffalo FPr amino acid sequence possesses a high degree of identity with the other mammalian homologues. Steady state concentration of buffalo FPr transcript increased (P > 0.05) from stage I to stage II/III, and declined at 18 h post $PGF_2_\alpha$ injection. The FPr concentration expressed as fmol/microg of plasma membrane protein showed an increase (P > 0.05) from stage I (1.98 \pm 0.10), through stage II/III (2.42 \pm 0.48) to stage IV (2.77 \pm 0.18). High affinity FPr was observed in stage I (K(d) 4.86 nmol) and stage II/III ($K_d$ 6.28 nmol) while low affinity FPr ($K_d$ 19.44 nmol) was observed in stage IV. In conclusion, we have cloned a full length FPr cDNA from buffalo cow CL and observed that FPr mRNA expression, receptor number and affinity did not vary significantly (P > 0.05) within the luteal phase of the oestrous cycle.

Item Type:	Journal Article
Publication:	Reproduction
Publisher:	Society for Reproduction and Fertility
Additional Information:	The water buffalo is the cornerstone of the livestock production-based agro-economy in many developing countries. We report for the very first time the cloning and expression of the buffalo FPr gene in the corpus luteum and it's expression pattern
Keywords:	Buffalo (Bubalus bubalis);corpus luteum;prostaglandin f2 alpha receptor
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	19 Feb 2007
Last Modified:	16 Jan 2013 11:51
URI:	http://eprints.iisc.ac.in/id/eprint/365

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