Localization and release of ?-glucosidase in the thermophilic and cellulolytic fungus, Sporotrichum thermophile

Joel Solomon , Gaikwad and Ramesh, Maheshwari (1994) Localization and release of ?-glucosidase in the thermophilic and cellulolytic fungus, Sporotrichum thermophile. In: Experimental Mycology, 18 (4). pp. 300-310.

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Abstract

In a medium containing cellulose as the carbon source, the rapid growth of Sporotrichum thermophile, the secretion of cellulases and the utilization of cellulose were well-correlated events. The production of beta-glucosidase in culture medium lagged behind cellulases, coinciding with the time of extensive autolysis of mycelia. By contrast, neither apparent autolysis nor secretion of beta-glucosidase occurred when S. thermophile was grown in medium containing cellobiose; the enzyme activity remained associated with mycelia. The release of beta-glucosidase in cellulose-grown cultures was correlated with the activity of the lytic enzyme in the cell wall. Immunocytochemical localization and biochemical characterization showed that a beta-glucosidase released in the cellulose medium was the same as that which remained associated with mycelia grown on cellobiose. The results indicated that the release of beta-glucosidase in the cellulose culture is incidental to the activity of the lytic enzymes which are strongly induced by cellulose. The observations minimize a functional role of the culture fluid beta-glucosidase in cellulolysis by the fungus. Rather, the available information suggests that the cellulases and beta-glucosidases associated with the hyphal cell wall may play a role in cellulolysis by the fungus. (C) 1994 Academic Press, Inc.

Item Type:	Journal Article
Publication:	Experimental Mycology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Sporotrichum thermophile;?-glucosidase;cellulolysis;lytic enzyme
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	26 Apr 2011 10:07
Last Modified:	26 Apr 2011 10:07
URI:	http://eprints.iisc.ac.in/id/eprint/36479

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