Excision of uracil from the ends of double stranded DNA by uracil DNA glycosylase and its use in high efficiency cloning of PCR products

Kumar, NV and Varshney, U (1994) Excision of uracil from the ends of double stranded DNA by uracil DNA glycosylase and its use in high efficiency cloning of PCR products. In: Current science (Bangalore), 67 (9-10). 728 -734.

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Abstract

We show that uracil DNA glycosylase from E. coli excises uracil residues from the ends of double stranded oligos. This information has allowed us to develop an efficient method of cloning PCR amplified DNA. In this report, we describe use of this method in cloning of E. coli genes for lysyl- and methionyl-tRNA synthetases. Efficiency of cloning by this method was found to be the same as that of subcloning of DNA restriction fragments from one vector to the other vector. Possibilities of using other DNA glycosylases for such applications are discussed.

Item Type:	Journal Article
Publication:	Current science (Bangalore)
Publisher:	Indian academy of sciences
Additional Information:	Copyright of this article belongs to Indian academy of sciences.
Department/Centre:	Division of Mechanical Sciences > Chemical Engineering
Date Deposited:	21 Apr 2011 06:10
Last Modified:	28 Nov 2018 15:14
URI:	http://eprints.iisc.ac.in/id/eprint/36463

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