Dendritic Poly(ether imine) Based Gene Delivery Vector

Thankappan, Ullas Padinjaremattathil and Madhusudana, Shampur Narayan and Desai, Anita and Jayamurugan, Govindasamy and Rajesh, Yamajala BRD and Jayaraman, Narayanaswamy (2011) Dendritic Poly(ether imine) Based Gene Delivery Vector. In: Bioconjugate Chemistry, 22 (2). pp. 115-119.

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Abstract

The nonviral vector based gene delivery approach is attractive due to advantages associated with molecular-level modifications suitable for optimization of vector properties. In a new class of nonviral gene delivery systems, we herein report the potential of poly(ether Mine) (PETIM) dendrimers to mediate an effective gene delivery function. PETIM dendrimer, constituted with tertiary amine branch points, n-propyl ether linkers and primary amines at their peripheries, exhibits significantly reduced toxicities, over a broad concentration range. The dendrimer complexes pDNA effectively, protects DNA from endosomal damages, and delivers to the cell nucleus. Gene transfection studies, utilizing a reporter plasmid pEGFP-C1 and upon complexation with dendrimer, showed a robust expression of the encoded protein. The study shows that PETIM dendrimers are hitherto unknown novel gene delivery vectors, combining features of poly(ethylene imine)-based polymers and dendrimers, yet are relatively nontoxic and structurally precise.

Item Type:	Journal Article
Publication:	Bioconjugate Chemistry
Publisher:	American Chemical Society
Additional Information:	Copyright of this article belongs to American Chemical Society.
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	23 Mar 2011 09:42
Last Modified:	23 Mar 2011 09:42
URI:	http://eprints.iisc.ac.in/id/eprint/36223

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