Patturajan, M and Chatterji, D and Rao, GR (1994) Accurate transcription initiation by RNA polymerase II from Candida utilis. In: Biochemistry & Molecular Biology International, 33 (5). pp. 901-907.
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An in vitro transcription system from Candida utilis is described. The template used is a hybrid plasmid containing Saccharomyces cerevisiae CYC1 promoter linked to a synthetic 377-bp G-minus casette (1). In vitro transcriptions are carried out in the presence of RNase. T1. Under these conditions only the transcripts that are resistant to RNase T1 accumulate. Using this protocol, it has been shown that in the absence of cytosolic factors RNA polymerase II (pol II) from C. utilis initiated RNA synthesis randomly. But both C. utilis and S. cerevisiae cell-free extracts could direct pol II from C. utilis to initiate transcription accurately. Results also indicated that the general transcription factors are functionally interchangeable between S. cerevisiae and C. utilis
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemistry & Molecular Biology International |
| Publisher: | Academic Press Aust |
| Additional Information: | Copyright of this article belongs to Academic Press Aust. |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 03 Jun 2011 07:30 |
| Last Modified: | 03 Jun 2011 07:30 |
| URI: | http://eprints.iisc.ac.in/id/eprint/36108 |
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