The Design and Construction of Synthetic Protein Mimics

Balaram, P (1993) The Design and Construction of Synthetic Protein Mimics. In: INTERNATIONAL SYMP ON ENZYMES IN ORGANIC SYNTHESIS, JAN 06-09, 1992 , NEW DELHI, INDIA.

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Abstract

A strategy for the modular construction of synthetic protein mimics based on the ability non-protein amino acids to act as stereochemical directors of polypeptide chain folding, is described. The use of alpha-aminoisobutyric acid (Aib) to construct stereochemically rigid helices has been exemplified by crystallographic and spectroscopic studies of several apolar peptides, ranging in length from seven to sixteen residues. The problem of linker design in elaborating alpha,alpha motifs has been considered. Analysis of protein crystal structure data provides a guide to choosing linking sequences. Attempts at constructing linked helical motifs using linking Gly-Pro segments have been described. The use of flexible linkers, like epsilon-aminocaproic acid has been examined and the crystallographic and solution state analysis of a linked helix motif has been presented. The use of bulky sidechain modifications on a helical scaffold, as a means of generating putative binding sites has been exemplified by a crystal structure of a peptide packed in a parallel zipper arrangement.

Item Type:	Conference Paper
Publisher:	National Institute of Science Communication and Information Resources
Additional Information:	Copyright of this article belongs to National Institute of Science Communication and Information Resources.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	10 Mar 2011 06:50
Last Modified:	10 Mar 2011 06:50
URI:	http://eprints.iisc.ac.in/id/eprint/35929

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