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Bacteriophage therapy for Staphylococcus aureus bacteremia in streptozotocin-induced diabetic mice

Sunagar, Raju and Patil, Shripad A and Chandrakanth, Kelmani R (2010) Bacteriophage therapy for Staphylococcus aureus bacteremia in streptozotocin-induced diabetic mice. In: Research in Microbiology, 161 (10). pp. 854-860.

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Official URL: http://dx.doi.org/10.1016/j.resmic.2010.09.011


The protective effect of bacteriophage was assessed against experimental Staphylococcus aureus lethal bacteremia in streptozotocin (STZ) induced-diabetic and non-diabetic mice. Intraperitoneal administrations of S. aureus (RCS21) of 2 x 10(8) CFU caused lethal bacteremia in both diabetic and non-diabetic mice. A single administration of a newly isolated lytic phage strain (GRCS) significantly protected diabetic and nondiabetic mice from lethal bacteremia (survival rate 90% and 100% for diabetic and non-diabetic bacteremic groups versus 0% for saline-treated groups). Comparison of phage therapy to oxacillin treatment showed a significant decrease in RCS21 of 5 and 3 log units in diabetic and nondiabetic bacteremic mice, respectively. The same protection efficiency of phage GRCS was attained even when the treatment was delayed up to 4 h in both diabetic and non-diabetic bacteremic mice. Inoculation of mice with a high dose (10(10) PFU) of phage GRCS alone produced no adverse effects attributable to the phage per se. These results suggest that phages could constitute valuable prophylaxis against S. aureus infections, especially in immunocompromised patients. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Item Type: Journal Article
Publication: Research in Microbiology
Publisher: Elsevier Science
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Diabetic complications; Multidrug resistance; Phage therapy; Staphylococcus aureus
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 22 Feb 2011 07:04
Last Modified: 22 Feb 2011 07:04
URI: http://eprints.iisc.ac.in/id/eprint/35672

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