Balakrishnan, Rajini A and Easwaran, KRK (1993) Lipid-amphotericin B complex structure in solution: A possible first step in the aggregation process in cell membranes. In: Biochemistry, 32 (15). 4139-4144 .
PDF
Lipid.pdf - Published Version Restricted to Registered users only Download (601kB) | Request a copy |
Abstract
The interactions between the polyene antibiotic amphotericin B with dipalmitoylphosphatidylcholine were investigated in vesicles (using circular dichroism) and in chloroform solution (using circular dichroism and IH, I3C, and 31P nuclear magnetic resonance). The results show that amphotericin B readily aggregates in vesicles and that the extent of aggregation depends on the 1ipid:drug concentration ratio. Introduction of sterol molecules into the membrane hastens the process of aggregation of amphotericin B. In chloroform solutions amphotericin B strongly interacts with phospholipid molecules to form a stoichiometric complex. The results suggest that there are interactions between the conjugated heptene stretch of amphotericin B and the methylene groups of lipid acyl chains, while the sugar moiety interacts with the phosphate head group by the formation of a hydrogen bond. A model is proposed for the lipid-amphotericin B complex, in which amphotericin B interacts equally well with the two lipid acyl chains, forming a 1:l complex.
Item Type: | Journal Article |
---|---|
Publication: | Biochemistry |
Publisher: | American Chemical Society |
Additional Information: | Copyright of this article belongs to American Chemical Society. |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
Date Deposited: | 11 Feb 2011 08:18 |
Last Modified: | 11 Feb 2011 08:18 |
URI: | http://eprints.iisc.ac.in/id/eprint/35529 |
Actions (login required)
View Item |