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Inhibition of in Vitro Aminoacylation and Translation by RNA Reactive Antibodies

Kala, Chandira K and Antony, A (1993) Inhibition of in Vitro Aminoacylation and Translation by RNA Reactive Antibodies. In: Immunological Investigations, 22 (2). pp. 117-126.

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Official URL: http://informahealthcare.com/doi/abs/10.3109/08820...


Antibodies were raised against guanosine-BSA, GMP-BSA and tRNA-mBSA conjugates separately in rabbits. Binding characteristics of these antibodies to various RNAs were studied using a sensitive avidin-biotin micro ELISA. These antibodies inhibited in vitro aminoacylation of tRNA in a dose dependent manner. This inhibition was reversed by the addition of the respective homologous haptens thereby showing the specificity of these antibodies. In vitro translation of endogenous mRNAs in rabbit reticulocyte lysate was also inhibited by these antibodies in a dose dependent manner.

Item Type: Journal Article
Publication: Immunological Investigations
Publisher: Informa plc
Additional Information: Copyright of this article belongs to Informa plc.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 10 Feb 2011 09:41
Last Modified: 10 Feb 2011 09:41
URI: http://eprints.iisc.ac.in/id/eprint/35508

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